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mouse anti wt1 (Santa Cruz Biotechnology)

Name: mouse anti wt1
Brand: Santa Cruz Biotechnology
Rating: 96 (1316 reviews)

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Santa Cruz Biotechnology mouse anti wt1
Mouse Anti Wt1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti wt1/product/Santa Cruz Biotechnology
Average 96 stars, based on 1316 article reviews

mouse anti wt1 - by Bioz Stars, 2026-02

96/100 stars

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( A ) Representative immunoblot images of profilin1 expression in primary podocytes freshly isolated from littermate control (Ctrl) and Pfn1 -KO mice (age P7). ( B ) Representative immunofluorescence images of profilin1 (green) and <t>WT1</t> (red) in control and Pfn1 -KO primary podocytes. Scale bar: 10 μm. ( C ) Representative immunofluorescence images of profilin1 (green) and nephrin (red) on kidney sections of control and Pfn1- KO mice (age 3 weeks). Scale bars: 10 μm. ( D ) Pfn1- KO mice (red) failed to gain body weight by 8 weeks of age compared with control mice (green). n = 9 mice. * P < 0.05 vs. control. ( E ) The survival curve of Pfn1- KO mice (red) demonstrates approximately 90% death by 12 weeks of age. n = 8 mice. ( F ) SDS-PAGE (Coomassie blue staining) of standard BSA and of urine samples from Pfn1 -KO mice at 4 weeks of age demonstrates albuminuria. Equal volumes of standard BSA and urine (4 μL) were loaded in each lane. ( G ) Quantification of urine albumin normalized to creatinine at 2, 6, and 10 weeks of age. n = 6 mice. * P < 0.05 vs. control. ( H ) Elevated plasma creatinine in Pfn1- KO mice at 3, 6, and 10 weeks of age. * P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

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Image Search Results

Journal: Cell reports

Article Title: A molecular atlas of the human postmenopausal fallopian tube and ovary from single-cell RNA and ATAC sequencing

doi: 10.1016/j.celrep.2022.111838

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse WT1 Clone 6F-H2 , Thermo Fisher Scientific , Cat#MA1–46028; RRID:AB_962464.

Techniques: Recombinant, Saline, RNAscope, Multiplex Assay, Diagnostic Assay, Software, Gene Expression, Microscopy, Real-time Polymerase Chain Reaction

Journal: Kidney360

Article Title: AMPK-SP1–Guided Dynein Expression Represents a New Energy-Responsive Mechanism and Therapeutic Target for Diabetic Nephropathy

doi: 10.34067/KID.0000000000000392

Figure Lengend Snippet: Antibodies and assay kits

Article Snippet: Mouse anti-WT1 , Novus Biologicals , NBP2-44607.

Techniques:

Journal: The Journal of Clinical Investigation

Article Title: Profilin1 is required for prevention of mitotic catastrophe in murine and human glomerular diseases

doi: 10.1172/JCI171237

Figure Lengend Snippet: ( A ) Representative immunoblot images of profilin1 expression in primary podocytes freshly isolated from littermate control (Ctrl) and Pfn1 -KO mice (age P7). ( B ) Representative immunofluorescence images of profilin1 (green) and WT1 (red) in control and Pfn1 -KO primary podocytes. Scale bar: 10 μm. ( C ) Representative immunofluorescence images of profilin1 (green) and nephrin (red) on kidney sections of control and Pfn1- KO mice (age 3 weeks). Scale bars: 10 μm. ( D ) Pfn1- KO mice (red) failed to gain body weight by 8 weeks of age compared with control mice (green). n = 9 mice. * P < 0.05 vs. control. ( E ) The survival curve of Pfn1- KO mice (red) demonstrates approximately 90% death by 12 weeks of age. n = 8 mice. ( F ) SDS-PAGE (Coomassie blue staining) of standard BSA and of urine samples from Pfn1 -KO mice at 4 weeks of age demonstrates albuminuria. Equal volumes of standard BSA and urine (4 μL) were loaded in each lane. ( G ) Quantification of urine albumin normalized to creatinine at 2, 6, and 10 weeks of age. n = 6 mice. * P < 0.05 vs. control. ( H ) Elevated plasma creatinine in Pfn1- KO mice at 3, 6, and 10 weeks of age. * P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Article Snippet: Rabbit anti-profilin1 monoclonal antibody (used for immunofluorescence, Thermo Fisher Scientific, catalog MA5-32683); rabbit anti-profilin1 polyclonal antibody (used for Western blotting, Cell Signaling Technology, catalog 3237S); guinea pig anti-nephrin polyclonal antibody (Progen, catalog GP-N2); rabbit anti-Wilms tumor 1 (WT1) monoclonal antibody (Abcam, catalog ab89901); mouse anti-WT1 monoclonal antibody (Novus Biologicals, catalog NB11-60011); rabbit anti-p21 monoclonal antibody (Abcam, catalog ab188224); mouse anti-p53 monoclonal antibody (Cell Signaling Technology, catalog 2524S); rabbit anti-cyclin monoclonal D1 antibody (Cell Signaling Technology, catalog 2978S); mouse anti-cyclin polyclonal B1 antibody (Cell Signaling Technology, catalog 4138S); mouse anti–Ser 319–phosphorylated γH2AX monoclonal antibody (EMD Millipore, catalog 05-636); rat anti-mCherry monoclonal antibody (Invitrogen, catalog M11217); goat anti-mVenus polyclonal antibody (MyBioSource, catalog MBS448126); Hoechst (Thermo Fisher Scientific, catalog 62249); rabbit anti-GAPDH monoclonal antibody (Cell Signaling Technology, catalog 2118S); mouse anti-GFP monoclonal antibody (Roche, catalog 11814460001); Alexa Fluor 488–conjugated phalloidin (Invitrogen, catalog A12379); Alexa Fluor 594–conjugated phalloidin (Invitrogen, catalog A12381); Alexa Fluor 488–conjugated tubulin (Abcam, catalog ab195883); Alexa Fluor 488 goat anti-mouse IgG antibody (Invitrogen, catalog A11029); Alexa Fluor 488 goat anti-rabbit IgG antibody (Invitrogen, catalog A11034); Alexa Fluor 488 donkey anti-goat IgG antibody (Invitrogen, catalog A11055); Alexa Fluor 594 goat anti-rabbit IgG antibody (Invitrogen, catalog A21207); Alexa Fluor 594 goat anti-mouse (Invitrogen, catalog A11032); Alexa Fluor 594 goat anti-guinea pig (Invitrogen, catalog A11076); Alexa Fluor 594 goat rat (Invitrogen, catalog A11007); Alexa Fluor 647 goat anti-rabbit (Invitrogen, catalog A21245); mouse anti–mouse IgG HRP-conjugated antibody (Rockland, catalog 18-8817-31); and rabbit anti–mouse IgG HRP-conjugated antibody (Millipore Sigma, catalog AP160P) were purchased commercially.

Techniques: Western Blot, Expressing, Isolation, Control, Immunofluorescence, SDS Page, Staining, Clinical Proteomics

( A ) Representative images of transmission electron micrography demonstrate abnormal MC podocytes in Pfn1 -KO mice (arrow) compared with control glomeruli at 4 weeks of age. Scale bar: 1 μm. ( B ) Immunofluorescence images of primary podocytes isolated from control and Pfn1 -KO mice at P7 stained with WT1 (red, podocyte marker) and Hoechst (blue, DNA marker) showing abnormal MC podocytes in Pfn1- KO mice (arrow) compared with control podocytes. Scale bar: 20 μm. ( C ) Immunofluorescence images of primary culture podocytes isolated from control and Pfn1 -KO mice stained with Hoechst and anti-tubulin antibody showing the chromosome bridge (arrow) in a MC Pfn1- KO podocyte. Scale bar: 20 μm. ( D ) Quantification of the percentage of MC podocytes per field of view in B . Total of 100 fields of view in 5 independent experiments. ( E ) Quantification of the percentage of chromosome bridge per field of view in C . n = 5 independent experiments. * P < 0.05 vs. control. ( F ) Immunofluorescence images of primary culture podocytes stained with γ-H2AX (green, double-strand breaks [DSBs] marker) and WT1 (red) showing abnormal MC podocytes in Pfn1- KO mice, as indicated by the arrows. Scale bar: 20 μm. ( G ) Quantification of γH2AX foci per podocyte nucleus (left) and per podocyte nuclear area (right) in F . Total of 400 cells in 5 independent experiments. * P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Journal: The Journal of Clinical Investigation

Article Title: Profilin1 is required for prevention of mitotic catastrophe in murine and human glomerular diseases

doi: 10.1172/JCI171237

Figure Lengend Snippet: ( A ) Representative images of transmission electron micrography demonstrate abnormal MC podocytes in Pfn1 -KO mice (arrow) compared with control glomeruli at 4 weeks of age. Scale bar: 1 μm. ( B ) Immunofluorescence images of primary podocytes isolated from control and Pfn1 -KO mice at P7 stained with WT1 (red, podocyte marker) and Hoechst (blue, DNA marker) showing abnormal MC podocytes in Pfn1- KO mice (arrow) compared with control podocytes. Scale bar: 20 μm. ( C ) Immunofluorescence images of primary culture podocytes isolated from control and Pfn1 -KO mice stained with Hoechst and anti-tubulin antibody showing the chromosome bridge (arrow) in a MC Pfn1- KO podocyte. Scale bar: 20 μm. ( D ) Quantification of the percentage of MC podocytes per field of view in B . Total of 100 fields of view in 5 independent experiments. ( E ) Quantification of the percentage of chromosome bridge per field of view in C . n = 5 independent experiments. * P < 0.05 vs. control. ( F ) Immunofluorescence images of primary culture podocytes stained with γ-H2AX (green, double-strand breaks [DSBs] marker) and WT1 (red) showing abnormal MC podocytes in Pfn1- KO mice, as indicated by the arrows. Scale bar: 20 μm. ( G ) Quantification of γH2AX foci per podocyte nucleus (left) and per podocyte nuclear area (right) in F . Total of 400 cells in 5 independent experiments. * P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Techniques: Transmission Assay, Control, Immunofluorescence, Isolation, Staining, Marker

( A ) Representative immunoblot images of cyclin D1, cyclin B1, P21, profilin1, and WT1 as loading control in control and Pfn1 -KO mouse primary podocytes. ( B ) Quantification of immunoblots in A . n = 5 independent experiments. ( C ) Representative immunofluorescence image of P21 expression in control and Pfn1- KO mouse glomeruli at 5 weeks of age stained with P21 (green) and WT1 (red). Scale bar: 20 μm. ( D ) Quantification of immunofluorescence intensity of nuclear P21 per podocyte in C . Total of 310 podocytes in 5 mice. ( E ) Representative immunofluorescence images of P53 in control and Pfn1- KO mouse glomeruli at 5 weeks of age stained with P53 (green) and WT1 (red). Scale bar: 20 μm. ( F ) Quantification of immunofluorescence intensity of nuclear P53 per podocyte in E . Total of 310 podocytes in 5 different mice. * P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Journal: The Journal of Clinical Investigation

Article Title: Profilin1 is required for prevention of mitotic catastrophe in murine and human glomerular diseases

doi: 10.1172/JCI171237

Figure Lengend Snippet: ( A ) Representative immunoblot images of cyclin D1, cyclin B1, P21, profilin1, and WT1 as loading control in control and Pfn1 -KO mouse primary podocytes. ( B ) Quantification of immunoblots in A . n = 5 independent experiments. ( C ) Representative immunofluorescence image of P21 expression in control and Pfn1- KO mouse glomeruli at 5 weeks of age stained with P21 (green) and WT1 (red). Scale bar: 20 μm. ( D ) Quantification of immunofluorescence intensity of nuclear P21 per podocyte in C . Total of 310 podocytes in 5 mice. ( E ) Representative immunofluorescence images of P53 in control and Pfn1- KO mouse glomeruli at 5 weeks of age stained with P53 (green) and WT1 (red). Scale bar: 20 μm. ( F ) Quantification of immunofluorescence intensity of nuclear P53 per podocyte in E . Total of 310 podocytes in 5 different mice. * P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Techniques: Western Blot, Control, Immunofluorescence, Expressing, Staining

( A ) A schematic representing cell cycle phase visualization by different colors in podocytes from the R26Fucci2aR Pfn1 -KO mice. G1 phase nuclei (mCherry, red); S phase nuclei (both mCherry and mVenus, yellow); and G2 phase nuclei (mVenus, green). ( B ) Representative immunofluorescence images of podocytes in control -FUCCI and Pfn1- KO- FUCCI glomeruli at 4 weeks of age stained with Cherry (red), Venus (green), and WT1 (blue). Scale bar: 20 μm. ( C ) Quantification of the distribution of podocytes in cell cycle phase in B . Total of 100 glomeruli in 5 different mice. ( D ) Primary podocytes isolated from control and Pfn1 -KO mice demonstrated a significant decrease in adhesion after plating for 5 minutes and 10 minutes on the collagen type I–coated plates. n = 6 independent experiments. ( E ) Representative immunofluorescence images of podocytes in control and Pfn1- KO glomeruli at 7 weeks of age stained with WT1 (red). Scale bar: 20 μm. ( F ) Quantification of podocyte density in glomeruli in E . Total of 40 glomeruli from 5 different mice. ( G ) Representative immunofluorescence images of urinary podocytes from control and Pfn1 -KO mice at 4 weeks of age stained with mCherry (red), mVenus (green), and WT1 (blue). G 1 phase podocyte nuclei (red arrow), S phase podocyte nuclei (yellow arrow), and G 2 phase podocyte nuclei (green arrow) were depicted. Scale bar: 20 μm. * P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Journal: The Journal of Clinical Investigation

Article Title: Profilin1 is required for prevention of mitotic catastrophe in murine and human glomerular diseases

doi: 10.1172/JCI171237

Figure Lengend Snippet: ( A ) A schematic representing cell cycle phase visualization by different colors in podocytes from the R26Fucci2aR Pfn1 -KO mice. G1 phase nuclei (mCherry, red); S phase nuclei (both mCherry and mVenus, yellow); and G2 phase nuclei (mVenus, green). ( B ) Representative immunofluorescence images of podocytes in control -FUCCI and Pfn1- KO- FUCCI glomeruli at 4 weeks of age stained with Cherry (red), Venus (green), and WT1 (blue). Scale bar: 20 μm. ( C ) Quantification of the distribution of podocytes in cell cycle phase in B . Total of 100 glomeruli in 5 different mice. ( D ) Primary podocytes isolated from control and Pfn1 -KO mice demonstrated a significant decrease in adhesion after plating for 5 minutes and 10 minutes on the collagen type I–coated plates. n = 6 independent experiments. ( E ) Representative immunofluorescence images of podocytes in control and Pfn1- KO glomeruli at 7 weeks of age stained with WT1 (red). Scale bar: 20 μm. ( F ) Quantification of podocyte density in glomeruli in E . Total of 40 glomeruli from 5 different mice. ( G ) Representative immunofluorescence images of urinary podocytes from control and Pfn1 -KO mice at 4 weeks of age stained with mCherry (red), mVenus (green), and WT1 (blue). G 1 phase podocyte nuclei (red arrow), S phase podocyte nuclei (yellow arrow), and G 2 phase podocyte nuclei (green arrow) were depicted. Scale bar: 20 μm. * P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test.

Techniques: Immunofluorescence, Control, Staining, Isolation

( A ) Representative immunofluorescence images of podocytes in control and Pfn1- KO glomeruli at 5 weeks of age stained with Rrp8 (green) and WT1 (red). Scale bar: 20 μm. ( B ) Quantification of immunofluorescence intensity of Rrp8 per podocyte in A . Total of 320 podocytes in 6 different mice. ( C ) Representative immunofluorescence images of podocytes isolated from control and Pfn1- KO mice at P7 stained with Rrp8 (red) and Hoechst (blue). Scale bar: 20 μm. ( D ) Quantification of immunofluorescence intensity of Rrp8 per podocyte in C . Total of 320 podocytes in 5 independent experiments. ( E and G ) Representative immunofluorescence images of podocyte isolated from Pfn1- KO mouse at P7 transduced with mouse Rrp8 lentiviral activation particles or control particles (Ctrl), followed by staining with Rrp8 (red) and Hoechst (blue), as shown in E , or γH2AX (green) and WT1 (red), as shown in G . Scale bar: 20 μm. ( F ) Quantification of the percentage of MC podocytes per field of view in E . Total of 100 fields of view in 5 independent experiments. ( H ) Quantification of γH2AX foci per podocyte nucleus (left) and per podocyte nuclear area (right) in G . Total of 400 cells in 5 independent experiments. * P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test. OE, overexpression.

Journal: The Journal of Clinical Investigation

Article Title: Profilin1 is required for prevention of mitotic catastrophe in murine and human glomerular diseases

doi: 10.1172/JCI171237

Figure Lengend Snippet: ( A ) Representative immunofluorescence images of podocytes in control and Pfn1- KO glomeruli at 5 weeks of age stained with Rrp8 (green) and WT1 (red). Scale bar: 20 μm. ( B ) Quantification of immunofluorescence intensity of Rrp8 per podocyte in A . Total of 320 podocytes in 6 different mice. ( C ) Representative immunofluorescence images of podocytes isolated from control and Pfn1- KO mice at P7 stained with Rrp8 (red) and Hoechst (blue). Scale bar: 20 μm. ( D ) Quantification of immunofluorescence intensity of Rrp8 per podocyte in C . Total of 320 podocytes in 5 independent experiments. ( E and G ) Representative immunofluorescence images of podocyte isolated from Pfn1- KO mouse at P7 transduced with mouse Rrp8 lentiviral activation particles or control particles (Ctrl), followed by staining with Rrp8 (red) and Hoechst (blue), as shown in E , or γH2AX (green) and WT1 (red), as shown in G . Scale bar: 20 μm. ( F ) Quantification of the percentage of MC podocytes per field of view in E . Total of 100 fields of view in 5 independent experiments. ( H ) Quantification of γH2AX foci per podocyte nucleus (left) and per podocyte nuclear area (right) in G . Total of 400 cells in 5 independent experiments. * P < 0.05 vs. control. Statistics were analyzed via a 2-tailed t test. OE, overexpression.

Techniques: Immunofluorescence, Control, Staining, Isolation, Transduction, Activation Assay, Over Expression

( A ) The percentage of patients with MC podocytes observed in kidney biopsy specimens using transmission electron microscopy (TEM) in patients with nonglomerular disease following nephrectomy (control; 0 of 5 patients, 0%), patients with focal segmental glomerulosclerosis (FSGS; 3 of 20 patients, 15%), patients with diabetic kidney disease (DKD; 3 of 26 patients, 11.5%), patients with primary membranous nephropathy (pMN; 16 of 145 patients, 11%), patients with lupus nephritis (LN; 3 of 33 patients, 9.1%), and patients with IgA nephropathy (IgAN; 7 of 110 patients, 6.4%) hospitalized between September 2019 and August 2022. ( B ) Representative TEM images of kidney biopsy specimens. Mononucleated podocytes in control and MC podocytes in patients with FSGS, DKD, pMN, LN, and IgAN are depicted with arrows. Scale bar: 2 μm. ( C ) Representative immunofluorescence images of glomeruli in control patients and patients with FSGS, DKD, pMN, LN, and IgAN with MC podocytes observed with TEM stained with profilin1 (green) and nephrin (red). Scale bar: 20 μm. ( D ) Quantification of C examining mean immunofluorescence intensity of profilin1 in the glomeruli. Total of 17 glomeruli in 3 patients from each group. * P < 0.05. Statistics were analyzed via a 1-way ANOVA with Dunnett’s correction. ( E ) Representative immunofluorescence images of urine samples in patients with FSGS, DKD, pMN, LN, and IgAN with MC podocytes stained with WT1 (green) and DAPI (blue). Arrows denote multinuclei. Scale bar: 20 μm. ( F ) Quantification of urine protein excretion in patients with FSGS, DKD, pMN, LN, and IgAN at the time of kidney biopsy. MC podocytes and non-MC podocytes were observed with TEM individually in each group.* P < 0.05. Statistics were analyzed via a 2-tailed t test.

Journal: The Journal of Clinical Investigation

Article Title: Profilin1 is required for prevention of mitotic catastrophe in murine and human glomerular diseases

doi: 10.1172/JCI171237

Figure Lengend Snippet: ( A ) The percentage of patients with MC podocytes observed in kidney biopsy specimens using transmission electron microscopy (TEM) in patients with nonglomerular disease following nephrectomy (control; 0 of 5 patients, 0%), patients with focal segmental glomerulosclerosis (FSGS; 3 of 20 patients, 15%), patients with diabetic kidney disease (DKD; 3 of 26 patients, 11.5%), patients with primary membranous nephropathy (pMN; 16 of 145 patients, 11%), patients with lupus nephritis (LN; 3 of 33 patients, 9.1%), and patients with IgA nephropathy (IgAN; 7 of 110 patients, 6.4%) hospitalized between September 2019 and August 2022. ( B ) Representative TEM images of kidney biopsy specimens. Mononucleated podocytes in control and MC podocytes in patients with FSGS, DKD, pMN, LN, and IgAN are depicted with arrows. Scale bar: 2 μm. ( C ) Representative immunofluorescence images of glomeruli in control patients and patients with FSGS, DKD, pMN, LN, and IgAN with MC podocytes observed with TEM stained with profilin1 (green) and nephrin (red). Scale bar: 20 μm. ( D ) Quantification of C examining mean immunofluorescence intensity of profilin1 in the glomeruli. Total of 17 glomeruli in 3 patients from each group. * P < 0.05. Statistics were analyzed via a 1-way ANOVA with Dunnett’s correction. ( E ) Representative immunofluorescence images of urine samples in patients with FSGS, DKD, pMN, LN, and IgAN with MC podocytes stained with WT1 (green) and DAPI (blue). Arrows denote multinuclei. Scale bar: 20 μm. ( F ) Quantification of urine protein excretion in patients with FSGS, DKD, pMN, LN, and IgAN at the time of kidney biopsy. MC podocytes and non-MC podocytes were observed with TEM individually in each group.* P < 0.05. Statistics were analyzed via a 2-tailed t test.

Techniques: Transmission Assay, Electron Microscopy, Control, Immunofluorescence, Staining